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Small RNA analysis using the Genome Sequencer™ FLX system

Nature Methods volume 4, pages v–vi (2007)Cite this article

Abstract

The Genome Sequencer™ FLX System from 454 Life Sciences™ and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, metagenomics analysis and amplicon sequencing. The Genome Sequencer FLX is built upon 454 Sequencing™ technology. One application in which the technology has had a considerable impact is small RNA analysis.

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Figure 1: The Genome Sequencer FLX Instrument.
Figure 2: One fragment = one bead = one read.
Figure 3: Before emPCR and sequencing, Genome Sequencer process–specific A and B adaptors must be appended to the cDNA copy of the small RNA.

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Authors and Affiliations

  1. 454 Life Sciences, 20 Commercial Street, Branford, 06405, Connecticut, USA

    Thomas Jarvie

  2. Roche Diagnostics, Roche Applied Science, 9115 Hague Road, Indianapolis, 46250, Indiana, USA

    Timothy Harkins

Authors
  1. Thomas Jarvie
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  2. Timothy Harkins
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Correspondence to Thomas Jarvie.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Jarvie, T., Harkins, T. Small RNA analysis using the Genome Sequencer™ FLX system. Nat Methods 4, v–vi (2007). https://doi.org/10.1038/nmeth1029

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