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Functional studies of microRNA based on knockdown using Locked Nucleic Acid probes

Nature Methods volume 4, pages iii–iv (2007)Cite this article

Abstract

Here we present a microRNA (miRNA) knockdown application, based on the use of Locked Nucleic Acid (LNA™)-modified probes. The use of LNA for the knockdown of miRNA is particularly effective because these probes have high affinity for their short RNA targets and excellent mismatch-discrimination capability. Here we demonstrate that LNA-modified probes, termed miRCURY™ LNA Knockdown probes, are highly specific and potent tools for functional analysis of miRNAs.

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Figure 1: Representative knockdown of microRNAs by miRCURY LNA Knockdown probes.
Figure 2: Mismatch discrimination ability of miRCURY LNA Knockdown probes.
Figure 3: Comparison of the effectiveness of different knockdown methods.

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  1. Exiqon, Bygstubben 9, Vedbæk, DK-2950, Denmark

    Søren Rasmussen & Peter Roberts

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  1. Søren Rasmussen
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  2. Peter Roberts
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Correspondence to Peter Roberts.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Rasmussen, S., Roberts, P. Functional studies of microRNA based on knockdown using Locked Nucleic Acid probes. Nat Methods 4, iii–iv (2007). https://doi.org/10.1038/nmeth1034

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