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MALDI tissue imaging: mass spectrometric localization of biomarkers in tissue slices

Nature Methods volume 4, page 462 (2007)Cite this article

Abstract

New mass-spectrometric proteomics technologies have been developed with the potential to transition from pure research applications into the diagnostic laboratory. Of these, one very promising technique has already gained much interest in the research community: matrix-assisted laser desorption/ionization (MALDI) molecular imaging. For easy and successful application of MALDI imaging in the laboratory, Bruker Daltonics offers an innovative product line: a stand-alone automated sample preparation device, ImagePrep™; mass spectrometers with vital smartbeam™ laser technology: namely, the autoflex III™ and ultraflex III™ MALDI–tandem time-of-flight (MALDI-TOF/TOF) instruments; and sophisticated software tools for fast data evaluation and biomarker visualization, flexImaging™.

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Figure 1: From tissue samples to biomarker visualization—the complete MALDI imaging workflow.
Figure 2: Analysis of a tissue slice of rat cerebellum.
Figure 3: Amyloid plaques in rat brain.

References

  1. Holle, A., Haase, A., Kayser, M. & Hoehndorf, J. Optimizing UV laser focus profiles for improved MALDI performance. J. Mass Spectrom. 41, 705–716 (2006).

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Acknowledgements

We thank M. Riemenschneider (Laboratory of Neurochemistry and Neurogenetics, Department of Psychiatry and Psychotherapy, Klinikum Rechts der Isar, Munich, Germany) for the Alzheimer model brain sections.

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Authors and Affiliations

  1. Bruker Daltonik GmbH, Fahrenheitstrasse 4, Bremen, 28359, Germany

    Martin Schuerenberg, Christine Luebbert, Sören-Oliver Deininger, Ralf Ketterlinus & Detlev Suckau

Authors
  1. Martin Schuerenberg
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  2. Christine Luebbert
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  3. Sören-Oliver Deininger
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  4. Ralf Ketterlinus
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  5. Detlev Suckau
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Corresponding author

Correspondence to Detlev Suckau.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Schuerenberg, M., Luebbert, C., Deininger, SO. et al. MALDI tissue imaging: mass spectrometric localization of biomarkers in tissue slices. Nat Methods 4, 462 (2007). https://doi.org/10.1038/nmeth1039

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