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Rapid automated selection of mammalian cell colonies by cell surface protein expression

Nature Methods volume 4, page 462 (2007)Cite this article

Abstract

Genetix's unique ClonePix FL technology uses our ability to grow mammalian cells into clonal colonies suspended in semisolid media, and image these colonies using fluorescence assays and markers. Here we describe a new application for this technology in the isolation of clonal cell populations based on their expression of cell surface proteins.

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Figure 1: Detection and selection of colonies of cells expressing endogenous receptors.
Figure 2: Detection and selection of colonies of CHO K-1 cells stably transfected with a construct encoding the human G protein–coupled purinergie receptor P2Y1.
Figure 3: Detection and selection of colonies of CHO K-1 cells that have been stably transfected with a Flag-tagged human drug target receptor.

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  1. Genetix Ltd., Queensway, New Milton, BH25 5NN, Hampshire, UK

    Edmund N C Newman & Dominick Whitney

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  1. Edmund N C Newman
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  2. Dominick Whitney
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Correspondence to Edmund N C Newman.

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Newman, E., Whitney, D. Rapid automated selection of mammalian cell colonies by cell surface protein expression. Nat Methods 4, 462 (2007). https://doi.org/10.1038/nmeth1047

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