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Stem and iPS cell selection: quantitation of surface marker (SSEA1) and intracellular GFP

Nature Methods volume 4, pages i–ii (2007)Cite this article

Abstract

Consistent and reliable propagation of undifferentiated stem cells is of particular interest in cell line development and drug screening. The Genetix ClonePix FL technology, already established for the rapid one-step identification and isolation of hybridomas and cell lines for biopharmaceutical production, can be used to isolate and collect stem cells expressing specific cell surface and intracellular proteins.

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Figure 1: CGR8 mES cells grown in CloneMatrix-based semisolid medium for 4 d and imaged for rhodamine-conjugated anti-SSEA1 on ClonePix FL.
Figure 2: Detection and picking of adherent MCF-7 clonal colonies expressing highest level of GFP.
Figure 3: Screen shot of graphical analysis generated by ClonePix FL to assist in the decision as to which clones to pick.

References

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Authors and Affiliations

  1. Genetix Ltd., Queensway, New Milton, BH25 5NN, Hampshire, UK

    Christopher J Mann, Edmund N C Newman, Dominick J Whitney, Nicola J Latchem, Irene Bramke & Julian F Burke

Authors
  1. Christopher J Mann
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  2. Edmund N C Newman
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  3. Dominick J Whitney
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  4. Nicola J Latchem
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  5. Irene Bramke
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  6. Julian F Burke
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Correspondence to Christopher J Mann.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Mann, C., Newman, E., Whitney, D. et al. Stem and iPS cell selection: quantitation of surface marker (SSEA1) and intracellular GFP. Nat Methods 4, i–ii (2007). https://doi.org/10.1038/nmeth1074

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  • DOI: https://doi.org/10.1038/nmeth1074

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