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HTRF® KinEASE™ TK: a new solution for tyrosine kinase screening

Abstract

Cisbio's HTRF® KinEASE™ assay for screening serine/threonine kinases now extends to tyrosine kinases (TK). The assay, developed in partnership with Millipore, uses a unique substrate containing a single phosphorylation site recognized by europium cryptate (Eu(K))− labeled antibody to phosphotyrosine. More than 60 tyrosine kinases have already been successfully tested with KinEASE TK. HTRF KinEASE TK kit is available in small and jumbo sizes for high-throughput screening and profiling applications.

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Figure 1: All assays based on HTRF KinEASE involve two steps: the enzymatic step and the detection step with HTRF reagents.
Figure 2: SEB titration on an Abl assay using HTRF KinEASE.

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Correspondence to Jean-Luc Tardieu.

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Tardieu, JL. HTRF® KinEASE™ TK: a new solution for tyrosine kinase screening. Nat Methods 4, i–ii (2007). https://doi.org/10.1038/nmeth1082

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