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Highlighting protein movement in living cells

Nature Methods volume 1, page 96 (2004)Cite this article

Abstract

A new photoactivatable fluorescent protein combines some of the best features of existing proteins used for in vivo labeling.

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Figure 1: A living endothelial cell transiently expressing the human dopamine transporter (hDAT) fused with the new photoswitchable cyan fluorescent protein (PS-CFP).

RESEARCH PAPERS

  1. Chudakov, D.M. et al. Photoswitchable fluorescent label for protein tracking. Nat. Biotechnol. published online 17 October 2004 (doi:10.1038/nbt1025).

  2. Patterson, G.H. & Lippincott-Schwartz, J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297, 1873–1877 (2002).

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  3. Chudakov, D.M. et al. Kindling fluorescent proteins for precise in vivo photolabeling. Nat. Biotechnol. 21, 191–194 (2003).

    Article  CAS  Google Scholar 

  4. Ando, R. et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 12651–12656.

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Authors
  1. Daniel Evanko
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Evanko, D. Highlighting protein movement in living cells. Nat Methods 1, 96 (2004). https://doi.org/10.1038/nmeth1104-96a

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  • DOI: https://doi.org/10.1038/nmeth1104-96a

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