Figure 3: Photo-cross-linking of Grb2(pBpa111) with the EGF receptor. | Nature Methods

Figure 3: Photo-cross-linking of Grb2(pBpa111) with the EGF receptor.

From: Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid

Figure 3

(a,b) The wild-type Grb2 or Grb2(pBpa111) was expressed together with the EGF receptor in CHO cells, treated or not treated with EGF, and excited with 365-nm light. The light exposure was maintained for 0, 5, 15 and 30 min. An immunoprecipitate with the anti-Flag antibody from the cell lysate (0.2 mg) was probed with the anti–EGF receptor antibody (a) and the anti-Flag antibody (b).

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