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MPep MALDI Chips for high-sensitivity and high-throughput peptide analysis by MALDI-TOF MS

Nature Methods volume 2, page 478 (2005)Cite this article

Abstract

In mass spectrometry, the need is rapidly growing for higher-sensitivity detection of low-abundance peptides, and for systems that use an automated, high-throughput workflow that includes offline liquid chromatography matrix-assisted laser desorption and ionization (LC-MALDI) coupling. SuNyx provides a new universal MALDI chip platform, which uses matrix spots deposited by vacuum sublimation through masks onto extremely low-wetting surfaces to provide superior control of liquid handling and sample spot preparation.

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Figure 1: Examples of MPep MALDI chips with different patterns of matrix.
Figure 2: Peak intensities of MALDI mass spectra depend on the liquid volume used for sample spot preparation.
Figure 3: Ultimate detection sensitivity of peptides using MPep MALDI chips.

References

  1. Muscate-Magnussen, A. et al. Proc. ASMS Conf. Mass Spectrom., ThPJ 162 (2004).

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Acknowledgements

The authors thank R. Swart at LC Packings/Dionex (Amsterdam, The Netherlands), as well as M. Glückmann, D. Waidelich and D. Merkel at Applied Biosystems (Darmstadt, Germany) for their continuous support in diverse technical aspects, P. Savickas formerly at Applied Biosystems (Framingham, Massachusetts, USA) for many helpful discussions as well as J. Pickartz and J. Wesener at Bayer Industry Services (Leverkusen, Germany) for taking measurements and instrumental support.

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Authors and Affiliations

  1. SuNyx GmbH, Stolberger Str. 370, Köln, DE-50933, Germany

    Holger Röhl, Sven Goethel & Karsten Reihs

Authors
  1. Holger Röhl
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  2. Sven Goethel
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  3. Karsten Reihs
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Corresponding author

Correspondence to Karsten Reihs.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Röhl, H., Goethel, S. & Reihs, K. MPep MALDI Chips for high-sensitivity and high-throughput peptide analysis by MALDI-TOF MS. Nat Methods 2, 478 (2005). https://doi.org/10.1038/nmeth766

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