Abstract
Sidec electron tomography (SET) is an innovative approach to three-dimensional (3D) protein visualization. SET uses low-dose radiation to produce images of individual proteins or macromolecular complexes—including integral membrane proteins—in their cellular context.
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References
Wartiovaara, J. et al. Nephrin strands contribute to a porous slit diaphragm scaffold as revealed by electron tomography. J. Clin. Invest. 114, 1475–1483 (2004).
Banyay, M. et al. Three-dimensional imaging of in situ specimens with low-dose electron tomography to analyze protein conformation. Assay Drug Dev. Technol. 2, 561–567 (2004).
Acknowledgements
SET was developed from research conducted at the Karolinska Institute in Stockholm, Sweden, and is commercially available through Sidec Molecular Imaging.
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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.
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Gadnell, M., Gunnarsson, K. Sidec electron tomography: bridging the gap between light microscopy and atomic structure determinations. Nat Methods 2, 632 (2005). https://doi.org/10.1038/nmeth781
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DOI: https://doi.org/10.1038/nmeth781
