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Pyro Q-CpG™: quantitative analysis of methylation in multiple CpG sites by Pyrosequencing®

Nature Methods volume 2, pages i–ii (2005)Cite this article

Abstract

Pyro Q-CpG from Biotage gives a new dimension to DNA methylation studies by quantitatively measuring the individual degree of methylation of consecutive CpG sites consistently over time. This reveals previously unseen patterns of methylation.

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Figure 1: The principle of Pyrosequencing and the output Pyrogram™.
Figure 2: Principle of analysis.
Figure 3: Data output from analysis of seven CpG sites in the p16INK4A promoter.

References

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Authors and Affiliations

  1. Biotage AB, Kungsgatan 76, Uppsala, 753 18, Sweden

    Robert England & Monica Pettersson

Authors
  1. Robert England
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  2. Monica Pettersson
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Correspondence to Monica Pettersson.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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England, R., Pettersson, M. Pyro Q-CpG™: quantitative analysis of methylation in multiple CpG sites by Pyrosequencing®. Nat Methods 2, i–ii (2005). https://doi.org/10.1038/nmeth800

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