Abstract
Here we describe a new array system (miRCURYTM LNA Array) for global microRNA (miRNA) profiling of biological samples. The miRCURY LNA Array system uses locked nucleic acid (LNATM), a high-affinity nucleotide analog, to enhance the array capture probes, giving them high sensitivity and excellent selectivity for miRNA targets. We have simultaneously developed a straightforward method for labeling miRNAs in a sample. As a result, the miRCURY LNA Array system can provide sensitiveand accurate profiling of miRNAs while requiring less sample and keeping sample handling stepsto a minimum.
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References
Castoldi, M. et al. A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA). RNA (in the press).
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Roberts, P. MicroRNA expression profiling on arrays enhanced with locked nucleic acids. Nat Methods 3, iii–iv (2006). https://doi.org/10.1038/nmeth869
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DOI: https://doi.org/10.1038/nmeth869
