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Analysis of small RNAs with the Agilent 2100 Bioanalyzer

Nature Methods volume 3, page 658 (2006)Cite this article

Abstract

The Agilent 2100 Bioanalyzer offers advantages of sensitivity and accuracy for performing RNA separation, detection and quantitation, coupled with a rapid, automated system. Here we demonstrate the performance of the Agilent 2100 Bioanalyzer compared with standard techniques for RNA separation, detection and quantitation.

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Figure 1: Comparison of RNA separation by agarose gel electrophoresis against the Agilent 2100 Bioanalyzer analysis.
Figure 2: Polyacrylamide gel analysis of RNAs.

References

  1. Zamore, P.D. & Haley, B. Ribo-gnome: the big world of small RNAs. Science 309, 1519–1524 (2005).

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Acknowledgements

The authors thank V. Caputo (CSS-Mendel Institute and Scientific Institute Casa Sollievo della Sofferenza), S. Prudente (CSS-Mendel Institute and Scientific Institute Casa Sollievo della Sofferenza), A. Pizzuti (CSS-Mendel Institute and University La Sapienza), V. Trischitta (CSS-Mendel Institute and University La Sapienza), B. Dallpiccola (CSS-Mendel Institute and University La Sapienza) and G. Franco Bottazzo (Bambino Gesú Children's Hospital) for fruitful discussions and suggestions.

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Authors and Affiliations

  1. Gene Expression and Microarrays Laboratory, Bambino Gesu Children's Hospital, Rome, Italy

    Andrea Masotti

  2. Agilent Technologies, Hewlett Packard Strasse 8, Waldbronn, 76337, Germany

    Tobias Preckel

Authors
  1. Andrea Masotti
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  2. Tobias Preckel
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Correspondence to Tobias Preckel.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Masotti, A., Preckel, T. Analysis of small RNAs with the Agilent 2100 Bioanalyzer. Nat Methods 3, 658 (2006). https://doi.org/10.1038/nmeth908

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