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Faster than real-time: confocal linescan systems provide ideal conditions for millisecond-resolution physiological imaging

Abstract

The LSM 5 LIVE is a new design for fast confocal imaging, which acquires images at extremely high frame rates and allows experiments not possible in the past, while maintaining the advantages of conventional scanning systems, such as adjustable confocal apertures and scalable frame formats with pan and zoom. Additionally, the LSM 5 LIVE offers fast and sensitive parallel acquisition with charge-coupled device (CCD) detectors for high quantum efficiency.

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Figure 1: LSM 5 LIVE scanhead design.
Figure 2: The scanning principle of LSM 5 LIVE.
Figure 3: Imaging and analysis with the LSM 5 LIVE.

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Engelmann, R. Faster than real-time: confocal linescan systems provide ideal conditions for millisecond-resolution physiological imaging. Nat Methods 3, III–V (2006). https://doi.org/10.1038/nmeth971

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