Table 1 Overview of the metabolic pathways included in the metabolic reconstructions.

From: The genome of Onchocerca volvulus, agent of river blindness

  

Number of reactions in pathway

Number of reactions predicted essential*

Superpathway

Metabolic pathway

L. loa

Ov and wOv

wOv only

L. loa

Ov and wOv

wOv only

Amino acid metabolism

Alanine, aspartate and glutamate metabolism

14

15

3

1

1

0

 

Arginine and proline metabolism

26

25

2

3

3

0

 

Biosynthesis of amino acids

37

41

9

3

3

0

 

Cysteine and methionine metabolism

17

19

2

4

4

0

 

Glycine, serine and threonine metabolism

17

17

3

1

1

0

Biosynthesis of other secondary metabolites

Streptomycin biosynthesis

4

4

0

2

2

0

Carbohydrate metabolism

Amino sugar and nucleotide sugar metabolism

23

23

3

4

4

0

 

Butanoate metabolism

8

8

0

1

0

0

 

Inositol phosphate metabolism

12

12

0

3

3

0

 

Pentose phosphate pathway

17

18

0

2

2

0

 

Propanoate metabolism

14

14

1

1

1

0

 

Pyruvate metabolism

20

20

1

1

1

0

Energy metabolism

Methane metabolism

16

16

0

2

2

0

Lipid metabolism

Arachidonic acid metabolism

16

14

0

3

3

0

 

Biosynthesis of unsaturated fatty acids

14

15

2

7

2

0

 

Fatty acid biosynthesis

3

15

32

1

1

0

 

Fatty acid degradation

32

32

0

19

0

0

 

Fatty acid elongation

28

28

0

24

0

0

 

Fatty acid metabolism

48

57

32

31

2

0

 

Glycerolipid metabolism

9

9

0

2

2

0

 

Glycerophospholipid metabolism

22

22

1

6

6

0

 

Sphingolipid metabolism

16

16

0

5

5

0

Metabolism of cofactors and vitamins

Nicotinate and nicotinamide metabolism†

11

12

1

1

0

1

 

One carbon pool by folate

10

10

6

1

0

0

 

Pantothenate and CoA biosynthesis

11

11

1

3

3

0

Metabolism of other amino acids

Beta-alanine metabolism

9

9

0

1

1

0

 

Glutathione metabolism

19

18

0

2

2

0

Metabolism of terpenoids and polyketides

Terpenoid backbone biosynthesis

12

12

2

8

8

0

 

Tetracycline biosynthesis

1

1

0

1

1

0

Nucleotide metabolism

Purine metabolism

53

63

9

7

3

0

 

Pyrimidine metabolism

47

51

7

7

5

0

Transport reactions

Extracellular transport

43

43

0

23

18‡

0

Total (non-redundant) number of reactions in pathways

428

455

72

86

49

1

Total number of reactions in reconstructions

777

796

100

112

70

1

  1. *One reaction was essential either from L. loa (with or without genetic evidence); O. volvulus (Ov) and Wolbachia (wOv) with reactions in the O. volvulus reconstruction (both from gene annotation and added in pathway gap-filling) not being contributed by wOv only; or wOv with reactions in the Ov reconstruction supported by genetic evidence only from wOv. †Only a single reaction, in the nicotinate and nicotinamide pathway, is essential and uniquely provided by wOv. Transport reactions for both nematode and Wolbachia lack genetic evidence therefore making wOv only and O. volvulus and wOv transporters indistinguishable. ‡Note that of the 18 transport reactions essential to O. volvulus, all are essential to L. loa as well except for threonine transport. Also reported is the total number of reactions belonging to the pathways listed here, as well as the total number of reactions in the reconstructions.
Back to article page