Supplementary Figure 8: The subtypes of neocortical interneurons in the adult human brain express transcription factors Sox6, COUP-TFII and Sp8.

(a–n) Brain sections from the parietal lobe of the adult neocortex immunostained with interneuron markers and transcription factors. Note that the majority of PV+ (a), CB+ (c), SOM+ (e) and nNOS+ (g) cells expressed Sox6. Few, if any, PV+ (b), CB+ (d) and NPY+ (i) cells expressed COUP-TFII or Sp8. A small number of SOM+ (f) and nNOS+ (h) cells in deeper layers expressed COUP-TFII. The majority of CR+ (m), VIP+ (n) and strongly Reelin+ (j) cells expressed COUP-TFII and/or Sp8. Few, if any, CR+ (l) and strongly Reelin+ (k) cells expressed Sox6. (o–t) Some interneuron markers were co-expressed in a subpopulation of neocortical interneurons. Note that a wide range overlap of SOM+, nNOS+ and CB+ cells (o). The majority of NPY+ cells expressed SOM and nNOS (p). Nearly all VIP+ cells expressed CR (t). However, no CR+/SOM+ (q) or CR+/nNOS+ (r) cells were found. Scale bars, 100 μm (a–t); 50 μm (inserts).