Supplementary Figure 1: Double labeled PV+/SOM+ O-A interneurons do not express Htr3a-GFP. | Nature Neuroscience

Supplementary Figure 1: Double labeled PV+/SOM+ O-A interneurons do not express Htr3a-GFP.

From: Dual origins of functionally distinct O-LM interneurons revealed by differential 5-HT3AR expression

Supplementary Figure 1

(a,b) Representative image of Parv+ (blue stain) and SOM+ (red stain) cells in O-A in the Htr3a-GFP and Nkx2.1-cre:RCE transgenic mouse lines. (c,d) Insets corresponding to regions delineated by dotted boxes in (a,b) showing the extent of co-localization of Htr3a-GFP- and NKX2.1Cre:RCE-expression in double labeled Parv+/SOM+ cells. (e) Histogram of the number of Htr3a-GFP- and Nkx2.1-cre:RCE -expressing cells that are Parv+/SOM+. Htr3a-GFP and Nkx2-1Cre:RCE data were derived from 3 separate mice each. 306-536 GFP+ O-A cells were counted from a total of 8–13 hippocampal sections per mouse. Box-and-whisker plots are constructed as follows; circle and line within box denotes mean and median, respectively; upper and lower limits of box and capped lines denote SEM and min/max data points, respectively.

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