Supplementary Figure 2: D1R does not regulate spine number.
From: Age-dependent regulation of synaptic connections by dopamine D2 receptors
(a, b) Cultured hippocampal neurons (DIV14) were transfected with the Venus construct along with either the empty vector or the D1R construct, and treated with vehicle or agonists and antagonists of D1R for 24 h. Representative images of transfected neurons (top) and dendrites at a higher magnification (bottom) are shown in (a). Quantification for (a) is shown in (b). (c) Quantification of total spine density in primary hippocampal neurons (DIV17) treated with vehicle, D2R agonist or D2R antagonist. The results were replicated by three independent experiments. Histograms show one of the three replicates (n = 15 neurons for each condition). Scale bar, 20 μM for images of neurons and 5 μM for images of dendrites. Mann Whitney test was used for statistical analysis. Data are presented as mean ± SEM.
