Supplementary Figure 4: BMP2-induced astroglial differentiation in p21 null mice is independent of Sox2-induced replicative stress.
From: Transcriptional repression of Bmp2 by p21Waf1/Cip1 links quiescence to neural stem cell maintenance
(a) Percentage of GFAP+ cells expressing high levels of Sox2 (Sox2high) amongst GFAP-Sox2 double positive cells, in saline (−) vs. Noggin (+) infused p21-null mice. F/DFn/Dfd: 17.30/2/2; P=0.1093. (b) Percentage of S100β/γH2AX double positive cells in wild-type and saline and noggin-infused p21-null animals. F/DFn/Dfd: 2.046/4/2; 12.63/2/1 P=0.7085; 0.3902. (c) Examples of the simultaneous detection of S100β and γH2AX in the SEZ of wild-type and saline and Noggin-infused p21-null animals. (d) Percentage of cells expressing high levels of Sox2 and (e) percentages of γH2AX and 53BP1 doubly positive cells in early and late-passage, wild-type and p21-null Noggin treated cells. (f) Fold change in neurosphere numbers produced by FACS-sorted cells from neurospheres infected with a retroviral construct for the expression of Sox2 (pMY-sox2) or empty vector (pMY), treated or not with 100 ng/ml Noggin. Data are shown as mean values ± s.e.m; *p<0.05. Scale bar in c: 35 μm.
