Supplementary Figure 3: Expression of Nestin and Math1 in purified CFP+ and GFP+ cells.
From: A population of Nestin-expressing progenitors in the cerebellum exhibits increased tumorigenicity
(a-d) Immediately after being isolated from the EGL of Math1-GFP/Nestin-CFP animals at P4, GFP+ cells (a) were stained for Math1 (red, b). Merged image of a and b indicates that all GFP+ cells express Math1 (c). GFP+ cells were also immunostained for Nestin (red, d). No Nestin expression was found among GFP+ cells. (e-g) CFP+ cells (e) from the EGL of Math1-GFP/Nestin-CFP cerebellum at P4 were immunostained for Nestin (red, f). The merged image of e and f shows Nestin expression in all purified CFP+ cells (g). CFP+ cells were immunostained for Math1 (red, h). No Math1+ cells were detected among CFP+ cell population. Scale bar: 200μm.
