Supplementary Figure 1: The gap junction blocker MFA abolishes electrical coupling.
From: Regulation of interneuron excitability by gap junction coupling with principal cells
a) Average traces from a coupled fusiform-stellate cell pair (upper and lower traces, respectively). Black traces are the baseline period and red traces are after 15-30 min of incubation in the gap junction blocker MFA (100-200 μM). The increased amplitude in the stellate cell prejunctional waveform after MFA likely reflects a contribution of gap junction channels to the basal leak conductance. b) Summary of the coupling coefficients from six pairs before and after application of MFA. Open circles represent individual experiments, red points are mean±1SEM. Left and right panels represent fusiform-to-stellate and stellate-to-fusiform directions, respectively. MFA significantly reduced the coupling coefficient in the fusiform-to-stellate cell direction from 0.10±10.02 to 0.008±1 0.002 (t(5)=4.74, p=0.005), whereas the stellate-to-fusiform coupling dropped from 0.018±10.003 to 0.002±10.001 (t(5)=4.63, 0.006, paired t-tests).
