Supplementary Figure 10: PV⁺ neuron activity regulates dendritic growth of newborn neurons during adult hippocampal neurogenesis.

(a) Presence of GABAergic synaptic inputs onto newborn neurons at 7 dpi from PV⁺ neurons. Same as in Fig. 1g, except that GFP⁺ newborn progeny at 4 and 7 dpi were examined and summaries for percentages of GFP⁺ cells recorded that exhibited PSCs (left), the mean amplitudes of PSCs (middle) and the PSC induction rate (right) are shown. The same data for 4 dpi as in Fig. 1g are shown for comparison. (b-f) Optogenetic manipulation of PV⁺ neuron activity affects dendritic development of newborn neurons. Shown in (b) is a schematic diagram of experimental procedure. Shown in (c) are sample projected confocal images of immunostaining for GFP and RFP. Scale bars: 20 mm. Also shown are summaries of total dendritic length (d) and branch numbers (e). Each dot represents data from one individual neuron (**: P < 0.01; *: P < 0.05; two sample Kolmogorov–Smirnov test). Shown in (f) is a Sholl analysis for dendritic complexity. Values represent mean ± s.e.m. (**: P < 0.01; two sample Kolmogorov–Smirnov test).