Supplementary Figure 3: The inhibitory effect of naspm on NAc MSN EPSC-70mV amplitude is not mediated by kainate receptors.
From: Synaptic depression via mGluR1 positive allosteric modulation suppresses cue-induced cocaine craving
The pharmacological action of naspm on glutamatergic synaptic transmission has been known to affect kainate receptor-mediated transmission (e.g., Sun H.Y. et al. (2009), J. Neurophysiology 101,1043-1055). We therefore conducted additional experiments in the presence of the selective kainate receptor antagonist UBP310 (5-10μM) and found that the inhibitory effect of naspm on MSN EPSC−70mV seen in brain slices obtained from “incubated rats” is indeed due to blockade of CP-AMPARs. (a) Timeline. SA, self-administration. (b) Naspm reduced EPSC−70mV amplitude to the same extent under control conditions (aCSF + naspm; 29.8 ± 3.3% reduction, 8 cells/5 rats; these data are also presented in Fig. 2c) and in the presence of UPB310 (UPB310 + naspm; 37.1 ± 6.2% reduction). n=5 cells/2 rats; t11=1.17, p=0.27, aCSF + naspm vs. UBP310 + naspm during last five minutes of naspm application.
