Supplementary Figure 7: No changes in total Homer protein levels are observed in the NAc during withdrawal from extended-access cocaine self-administration.
From: Synaptic depression via mGluR1 positive allosteric modulation suppresses cue-induced cocaine craving
(a) Timeline. SA, self-administration. (b) Homer1bc (left) and Homer2 (right) protein levels were assessed in total NAc tissue at each withdrawal day (WD) time-point (same rats as those used in Figs. 3 and 4 of main text), and no change in either isoform was observed. Data are shown as percent saline control at each time-point. Note that, as in Fig. 3, differences in signal intensity for saline groups on different withdrawal days reflect differences in exposure time for different blots rather than time-dependent changes in protein levels after saline self-administration. Homer1bc, WD14: saline n=10 rats, cocaine n=11 rats. Homer 2, WD14: saline n=10 rats, cocaine n=9 rats. Homer1bc, WD25: saline n=10 rats, cocaine, n=10 rats; Homer2, WD25: saline n=10 rats, cocaine n=9 rats; Homer1bc, WD48 and Homer2, WD48: saline n=9 rats, cocaine n=11 rats.
