Supplementary Figure 4: Schematic and validation of the retrograde AAV2/5-DIO-ChR2-eYFP in Thcre mice. | Nature Neuroscience

Supplementary Figure 4: Schematic and validation of the retrograde AAV2/5-DIO-ChR2-eYFP in Thcre mice.

From: Stress and CRF gate neural activation of BDNF in the mesolimbic reward pathway

Supplementary Figure 4

(a) Schematic of ChR2 into NAc and ferrule implantation into VTA of Thcre mice and timeline. (b) Confocal image showing co-expression of AAV2/5-DIO-ChR2-eYFP and TH-positive cells in VTA to NAc cells of Thcre (scale 25 μm). (c) Phasic light stimulation induced spiking in VTA-NAc DA neurons expressing AAV2/5.

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