Supplementary Figure 5: Reduction of growth of BTICs but not their differentiated progenies by monocytoid cells.
From: Therapeutic activation of macrophages and microglia to suppress brain tumor-initiating cells
The 25EF and 48EF BTIC lines were grown in BTIC medium or were differentiated by culture for 5d in 1% FBS medium. 104 cells/well were then exposed to MCM or AmpB-treated microglia conditioned medium (AmpB/MCM) from 2 separate microglia cultures (MCM1 or MCM2) for 72h, and the total number of cells/well was counted (a); panel b depicts representative images at 72h. Similar experiments were performed for medium conditioned by monocytes (c), or with co-culture with microglia (MG) (d). **p<0.01, ***p<0.001 compared to respective controls (1 way ANOVA) (n of 4 wells/histogram, Error bars, s.d.). The p value at the top of each set of histograms refers to the overall ANOVA value.
