Supplementary Figure 8: Specificity of P2ry12 and FCRLS antibodies in resident microglia.
From: Identification of a unique TGF-β–dependent molecular and functional signature in microglia
(a) Immonohistochemical analysis of mouse spinal cord, spleen red and pulp, lung, kidney, liver and skin (ear) stained with anti-P2ry12 (microglia; red), anti-Iba-1 (myeloid cells; green) and DAPI (nucleus; blue). Representative images of 3-5 mice. (b) FACS analysis of FCRLS surface expression in splenic CX3CR1GFP/+ monocytes and CD11b/CD45+ brain microglia. (c and d) Confocal images of mouse brain stained with Iba-1 and polyclonal FCRLS antibody in (c) naïve and (d) chimeric CX3CR1GFP/+ mice. Anti-FCRLS signal co-localized with Iba-1+ microglia and does not stain recruited monocytes in the brain of CX3CR1GFP chimeric mouse transplanted with bone marrow derived cells from CX3CR1GFP/+ transgenic mice.
