Supplementary Figure 9: Microglia signature during development.
From: Identification of a unique TGF-β–dependent molecular and functional signature in microglia
(a) MG400 expression profile of microglia from mice bran at E10.5, E12.5, P3, P21, P30 and 2 months of age (full gene list, Source data – Supplementary Figure 9). Results were log-transformed, normalized (to the mean expression of zero across samples) and centered, and populations and genes were clustered by pairwise centroid linkage with the Pearson correlation. Data are pooled of 2 different experiments (n = 5-10 pooled mice per group). (b) Top microglial molecules grouped according to cell localization and function. (c) qPCR analysis of 6 selected microglia genes in MCSF+TGF-β1 cultured microglia. Gene expression level was normalized against Gapdh using ΔCt.
