Supplementary Figure 12: Macrophages, dendritic cells and Langerhans cells are not affected in peripheral organs of CNS-TGFβ1−/− mice.
From: Identification of a unique TGF-β–dependent molecular and functional signature in microglia
(a-d) Nonlymphoid and lymphoid tissue myeloid cells isolated from IL2TGFβ1-Tg-TGF-β1+/− (left) and IL2TGFβ1-Tg-TGF-β1−/− (right) mice at 160d were analyzed by flow cytometry. FACS representative dot plots show the percentage and absolute numbers of (a) kidney, liver, lung, spleen and skin derived CD11b+F4/80+ macrophages and (b) Langerhans cell (LC) stained with MHCII+CD11b+ and Langerin+CD11b+ in the ear skin cell suspension among DAPI−CD45+ cells of IL2TGFβ1-Tg-TGF-β1+/− (n = 6) and IL2TGFβ1-Tg-TGF-β1−/− (n = 6) mice. (c) Plots show percentage of CD11c+ DCs among gated DAPI−CD3−CD19−NK1.1+ cells. (d) Dot plots show percentage of CD103+ and CD11b+ DCs among gated DAPI-CD45+CD11c+I-A+ cells. Bars represent data from 2 pooled experiments. Errors bars represent ±SEM.
