Supplementary Figure 7: LRRK2 regulates PKARIIβ dendritic spine localization.
From: LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity
(a-c) The full-length images of Supplementary Fig. 6c (a), 6d (b), and 6e (c). (d) Fluorescent images of GFP-PKACα and mCherry in dendrites of transfected Lrrk2+/+ and Lrrk2–/– hippocampal neurons at 15DIV. Scale bars: 5μm. (e) Representative image of cultured Lrrk2–/– hippocampal neurons co-transfected with GFP-PKARIIβ (green), mCherry (red) and myc-LRRK2 (blue). The e1 and e2 are enlarged images of the boxed areas in (e). Scale bars: 20μm (e), 20μm (e1), and 2μm (e2). (f) Representative images showing spines and their parental dendrites from cultured Lrrk2+/+ hippocampal neurons co-transfected with mCherry and either GFP-PKARIIb, GFP-PKARIIβΔ2-5 or GFP-PKARIIβΔ2-5MTBD. Scale bars: 2μm. (g) Images of cultured Lrrk2–/– hippocampal neuron co-transfected with mCherry and PKARIIβΔ2-5MTBD. Scale bars: 2μm. (h) The full-length images of Fig. 5d.
