Supplementary Figure 10: The full-length images of Fig. 7.
From: LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity
(a-e) The full-length images of Fig. 7a (a), 7c (b), 7g (c), 7i (d), and 7k (e). (f) Western blot shows the specificity of pS3 cofilin antibody. HEK293 cells were transfected with GFP-tagged wild-type (WT), S3A, and S3D mutant cofilin constructs. The pS3 cofilin antibody only recognized the GFP-WT cofilin but not the ones with mutations at the S3 residue. (g) Western blot shows the specificity of PKARIIb antibody. Mouse N2a cell lines were transfected with control and four independent PKARIIβ siRNA. A significant reduction of PKARIIb protein levels was found in all PKARIIβ siRNA-transfected cells. (h) Western blot shows the specificity of PKACα antibody. N2a cells were transfected with control and four independent PKACα siRNA. A significant reduction of PKACα protein levels was found in all PKACα siRNA-transfected cells.
