Supplementary Figure 1: LRRK2 developmentally regulates dendritic spine maturation and synaptic transmission
From: LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity
(a) Western blot analysis of PSD95 expression in the striatal extract of P2, P7, P15, P21, and P30 LRRK2+/+ and LRRK2–/– mice. Actin was used as the loading control. (b) Bar graph depicts the quantification of PSD95 levels (n=3 mice per genotype and per time point). Data are represented as mean ± SEM. Unpaired t-test, t(4)=0.0643, 0.3217, 3.270, 3.411, and 0.4935, respectively. *P < 0.05. (c-e) Whiskers blots show average dendritic spine density (c), head diameter (d) and length (e) of SPNs in 1-month-old Lrrk2+/+ and Lrrk2–/– mice. NLrrk2+/+=761 spines and NLrrk2–/–=749 spines were from six neurons of three mice per genotype. Data represent mean ± SEM. Unpaired t-test, t(1508)=10.43, ****P<0.0001.
