Supplementary Figure 8: Control experiment to demonstrate third ventricle cannulation.
From: Circadian behavior is light-reprogrammed by plastic DNA methylation
(a) Infusion of TGFα into the third ventricle is known to alter the locomotor activity of mice (Kramer et al., Science (2001) 294:2511-15). Bar graph showing average normalized 3-day activity before, during and after 7 days of infusion with aCSF or TGFα. Four-week-old mice were exposed to DD for 10 days to record their FRP and then infused with aCSF or TGFα for 7 days in DD via osmotic minipump, mean ±s.e.m. shown. (b) Coronal brain section from representative cannulated mice, showing the needle track towards the third ventricle.
