Supplementary Figure 3: mOP- and dOP-LTD are expressed presynaptically, whereas kOP-LTD has an unclear site of expression.
From: Opioids induce dissociable forms of long-term depression of excitatory inputs to the dorsal striatum
(a) Representative traces of sEPSCs recorded from a DLS MSN before, immediately after, and 20-25 min following DAMGO (0.3 μM, 5 min). DAMGO (0.3 μM, 5 min) induced a long-lasting increase in sEPSC inter-event interval (IEI) (b,c; P=0.0063, Friedman statistic=9.800, n=9) with no change in sEPSC amplitude (d; P=0.2223, Friedman statistic=3.200). (e) Representative sEPSC traces for DPDPE (0.3 μM, 5 min). DPDPE (0.3 μM, 5 min) produced a long-lasting increase in sEPSC IEI (f,g; P=0.0207, Friedman statistic=7.714, n=7) with no change in sEPSC amplitude (h; P=0.0854, Friedman statistic=5.429). (i) Representative sEPSC traces for U69,593 (0.3 μM, 5 min). U69,593 (0.3 μM, 5 min) produced no change in sEPSC IEI (j,k; P=0.0570, Friedman statistic=6.000, n=9), but produced a delayed decrease in sEPSC amplitude (l; P=0.0307, Friedman statistic=6.889). Scale bars: 20 pA, 2 s. Data in c-d, g-h, and k-l analyzed with Friedman test with Dunn's multiple comparisons post-test. *: P<0.05.
