Supplementary Figure 6: Inflammation in vivo in HD individuals.
From: Mutant Huntingtin promotes autonomous microglia activation via myeloid lineage-determining factors
qRT-PCR analysis for IRF1 (a) and TNFα (b) mRNAs expression in striatum (first column, n= 9 individual per group), cortex (second column, n= 9 individual per group) and monocytes (third column, n= 5 individual per group) from controls and HD individuals. Each dot is representative of one individual. All p values were determined by unpaired student t-test. (c) IHC controls: brain section in presence of rabbit IgG (negative control) (i); spleen section in presence of rabbit IgG (negative control) (ii); spleen section in presence of diluting buffer (BSA, 1% bovine serum albumin in PBS phosphate buffered saline) (iii); H&E staining on spleen section (iv): PU.1 IHC staining on spleen section (positive control) (v); Von Willebrand factor IHC staining on spleen section (positive control for endothelial cells/blood vessels) (vi). Scale bar: 100μm.
