Supplementary Figure 7: Dynamic-clamp stimulation of a CA1 pyramidal cell.
From: Unbalanced excitability underlies offline reactivation of behaviorally activated neurons
(a) Joint conductances of SW-relevant EPSC-like and IPSC-like waveforms were injected into a CA1 pyramidal cell under the pharmacological blockade of fast synaptic transmission. (b) Raw traces of the spike responses to the conductance injection in a representative CA1 neuron are shown with truncated spikes. The conductance peak amplitude was 0.53–4 nS for Ge(t) and 4 nS for Gi(t), whereas the time difference between Ge(t) and Gi(t) ranged from 0 to 6 ms. We tested 49 (= 7 × 7) combinations of EPSC and IPSC waveforms. (c) Pseudocolored map of the probability that CA1 pyramidal neurons fire spikes in response to dynamic-clamp conductance injection with various combinations of the EPSC-to-IPSC ratios and timings. Data were averaged from 9 neurons (20 trials each, from 5 slices in 5mice). (d) The same data shown in (c) are plotted as the means ± SD.
