Supplementary Figure 1: SWs in vitro.
From: Unbalanced excitability underlies offline reactivation of behaviorally activated neurons
(a) Representative LFP trace (top) and 120–250 Hz filtered trace (bottom) recordings from CA1 stratum pyramidale. SW timings are shown with blue lines. Three SWs are magnified in the left insets. The peak ripple oscillation frequency was 191 ± 22 Hz (mean ± SD). (b) The brains were sliced horizontally (top) or at an angle of 12.7° to the fronto-occipital axis (bottom). (c) CA3 stratum pyramidale was stimulated at various intensities, and neurons that fired in response to the stimulation were monitored in CA1 stratum pyramidale using functional multineuron calcium imaging with Oregon Green 488 BAPTA-1. The mean percentage of spiking neurons to the total neurons recorded in horizontal and oblique slices are plotted as a function of the stimulation intensity. These results imply that CA3-to-CA1 axonal projections were more preserved in oblique slices than in horizontal slices (**P = 6.6×10-5, F1,28 = 21.9, two-way ANOVA). Error bars are SDs of 4 or 5 slices from 3 mice. (d) Paired LFP recordings from CA3 and CA1. SWs in CA1 occurred together with CA3 network activity in an intact hippocampal slice. In slices that received surgical lesions between CA3 and CA1, CA3 network activity still remained, but SWs in CA1 disappeared. (n = 3 slices from 2 mice each).
