Supplementary Figure 5: Positive correlation between the membrane penetration activity of WT and mutant forms of syt and eEPSC amplitude at a lower concentration of Ca2+.
From: Linker mutations reveal the complexity of synaptotagmin 1 action during synaptic transmission
(a–b) Scatter plots of peak values of the normalized NBD fluorescence of C2A*(3)-C2B (a) and C2A-C2B*(b) in 100 μM Ca2+, using liposomes that harbored 25% PS. For each condition, 3 independent experiments were carried out. (c, d). Positive correlation between the membrane penetration activity of C2A (c), and C2B (d), and eEPSC amplitude recorded at 2 mM [Ca2+]o, taken from Supplemental Fig. 1b. The mean values ± SEM are indicated. Statistical significance was determined using a bootstrap approach. Asterisks indicate differences compared with WT; * p < 0.05, *** p < 0.001. Detailed statistical analysis, and the equations and p-values for the linear regressions, are provided in Supplementary Table 1.
