Supplementary Figure 1: The experiments in Fig. 2a, b, d and 3a, b were repeated at 2 mM [Ca2+]o.
From: Linker mutations reveal the complexity of synaptotagmin 1 action during synaptic transmission
(a) Representative eEPSC traces recorded from WT, syt KO, and KO neurons that expressed each linker mutant. (b, c) Scatter plots of single eEPSC amplitudes (b) and latencies to peak (c). (d) Representative eEPSCs recorded in paired-pulse experiments with 50 ms inter-stimulus intervals. (e) Scatter plot of the PPRs, calculated as in Fig. 3. For each condition, we recorded from 26–29 cells from a total of six coverslips, where two coverslips were obtained from each of three independent litters of mice. The mean values ± SEM are indicated. Bootstrap statistical analysis was carried out, and the results are provided in Supplementary Table 1. Asterisks indicate differences compared with WT; *** p < 0.001.
