Supplementary Figure 9: Characterization of MATR3 antibodies.
From: Mutations in the Matrin 3 gene cause familial amyotrophic lateral sclerosis
The specificity of MATR3 antibodies Abcam 151714 (a) and Sigma HPA0036564 (b) were tested against purified MATR3 protein (Origene TP323258), lysates from HEK293FT cells treated with siRNA (untransfected control, non-targeting control, Cyclophilin B and MATR3), and cells overexpressing MATR3(WT)-GFP. Both antibodies were specific to MATR3 as indicated by the MATR3 siRNA sample showing a reduction in MATR3 protein level compared to untransfected, non-targeting and Cyclophilin B controls.
