Supplementary Figure 2: Lumbar spinal cord tissue immunostained for MATR3 and counterstained with hematoxylin.
From: Mutations in the Matrin 3 gene cause familial amyotrophic lateral sclerosis
(a) Control spinal cord exhibits MATR3 nuclear immunoreactivity in some motor neurons, with weak glial cell immunostaining. (b) Spinal cord from a subject with ALS exhibits strong nuclear immunoreactivity, with cytoplasmic immunoreactivity present in some motor neurons either diffusely or in cytoplasmic puncta. Strong glial immunostaining is also noted in samples from ALS patients. (c) Spinal cord from a patient with the Phe115Cys MATR3 mutation exhibits strong nuclear staining, as well as cytoplasmic staining in many cells. Images were taken at 20x magnification; insets show enlargements of the boxed regions. Scale bars represent 50 μm.
