Supplementary Figure 6: Basic properties of medium spiny neurons and astrocytes. | Nature Neuroscience

Supplementary Figure 6: Basic properties of medium spiny neurons and astrocytes.

From: Astrocyte Kir4.1 ion channel deficits contribute to neuronal dysfunction in Huntington's disease model mice

Supplementary Figure 6

Medium spiny neurons (MSN) and astrocytes in the striatum were easily distinguished during whole-cell recordings with Alexa 488 fluorescent dye in the pipette solution. (a) Shows a typical dendritic morphology of a MSN dialyzed with Alexa 488 (50 μM) for 30 min and the electrophysiological traces below show a negative resting membrane potential (-81 mV) and action potential firing during small depolarizing current injections. (b) Shows an astrocyte identically dialyzed with Alexa 488: a few short processes can be seen with a generally bushy appearance. The electrophysiological traces below show a negative resting membrane potential (-80 mV), but no action potential firing even for large depolarizing current injections.

Back to article page