Supplementary Figure 3: Illustration of short RNA reads in adult mouse sperm. | Nature Neuroscience

Supplementary Figure 3: Illustration of short RNA reads in adult mouse sperm.

From: Implication of sperm RNAs in transgenerational inheritance of the effects of early trauma in mice

Supplementary Figure 3

(a) aligning to the mouse genome, (b) mapping to mature miRNA sequences (allowing for overhanging 5' and 3' nucleotides) and (c) aligning to piRNA clusters. In (a), reads alignment shows peaks at the typical size of miRNAs (21-23bp) and piRNAs (26-31bp). In (b), mapping of 18-35bp reads (not mapping to the transcriptome) to annotated miRNAs shows a sharp peak at 22bp, the typical size of mature miRNAs. In (c), alignment of 18-35bp reads (not mapping to the transcriptome) to genomic regions annotated as piRNAs shows a peak at the typical size of piRNAs, starting with the nucleotide T indicative for piRNA identity. A concatenation of all reads detected in control libraries is shown. The size and first nucleotide are shown by position on the x-axis and color, respectively. The y-axis shows the percentage of reads relative to total reads for the combined libraries.

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