Supplementary Figure 5: Experimental design for MSUS treatment and breeding.
From: Implication of sperm RNAs in transgenerational inheritance of the effects of early trauma in mice
C57Bl/6J females (F0, left) were bred to C57Bl/6J males and their pups were subjected to MSUS from postnatal day (PND) 1 to 14 or raised in normal conditions (Control). Males from the F1 offspring were then bred to naïve C57Bl/6J females to obtain second-generation animals (F2) that were raised in normal conditions (no maternal separation or maternal stress). Animals from F1 and F2 generations were tested behaviorally then bred. Illustration: University of Zürich informatics services, MELS, Natasa Milosevic.
