Supplementary Figure 5: Behavioral analysis of mice lacking MeCP2 in forebrain glutamatergic neurons.

(a) Percentage time spent in open and closed regions of elevated zero maze (p = 0.50). (b) Number of beam breaks across 5 minutes of open field assay (p = 0.043). (c) Percentage time spent in center and periphery of open field assay (p = 0.50). (d) Latency to fall from accelerating rotarod (F[1, 272] = 3.70, p = 0.056, genotype). (e) Social approach towards a social (mouse) versus a nonsocial object in a three-chamber test (F[1, 14] = 0.02, p = 0.89, genotype). (f) Social approach behavior towards a novel mouse versus a familiar cage mate control mouse in a three-chamber test (F[1, 12] = 0.78, p = 0.40, genotype). (g) Time spent freezing during fear-conditioning training following foot shocks (F[1, 51] = 1.57, p = 0.22, genotype). (h) Time spent freezing in response to context or cue 24 hours following training (F[1, 34] = 3.11, p = 0.09, genotype). Bars represent mean ± s.e.m. Statistics were performed using two-way ANOVA or an unpaired two-tailed t-test with Welch's correction. Behavioral experiments were performed at 13-15 weeks of age (n = 9 for Mecp2^2lox/y; Emx1-Cre mice and n = 10 for wild-type, Mecp2^2lox/y littermates).