Supplementary Figure 4: LC neurons express Nav1 and NALCN channels.
From: Mitochondrial oxidant stress in locus coeruleus is regulated by activity and nitric oxide synthase
(a) Box-plot quantification of relative mRNA abundance for Nav1 channels in SNc and LC (n=5 mice). (b) Box-plot quantification of relative mRNA abundance for NALCN channels in SNc and LC (n=5 mice). (c) Sample trace of pacemaking and spikelets (with TTX). (d) LC neurons after isradipine eliminates spikelets and membrane potential remains depolarized, and sodium replacement with N-methyl-D-Glucamine (NMDG) hyperpolarizes LC neurons, suggesting a strong contribution of NALCN channels in LC neurons. (e) Box-plot quantification of membrane potential under normal Na+ and equimolar substitution with NMDG (n=4 neurons from 4 mice, p=0.0284, Mann-Whitney).
