Supplementary Figure 5: Time course of Fluo4 dye loading in LC neurons synchronized to spike-evoked Ca2+ transients.
From: Mitochondrial oxidant stress in locus coeruleus is regulated by activity and nitric oxide synthase
(a) Representative time course plotting Fluo4 (200 μM in the pipette) fluorescence as a function of time after whole cell break in. Fluorescence was sampled in the soma. During the time course of dye loading, action potential spikes were evoked in silenced LC neurons (shown in inset), and evoked Ca2+ transients were analyzed for buffering capacity calculations until dye reached equilibrium (∼5-10 min for soma).
