Supplementary Figure 6: NOS inhibition inhibits NO production in LC neurons.
From: Mitochondrial oxidant stress in locus coeruleus is regulated by activity and nitric oxide synthase
(a) DAF-FM fluorescence was measured in LC neurons every five minutes, before and after bath application of 100 μM L-NAME (10 min). The increase fluorescence is an indicator of increased NO production, calculated by linear fit, giving rise to a slope that indicates rate of NO production. L-NAME decreased the rate of NO production, shown by a decrease in the slope (middle panel). (b) Quantification of rate of NO production (slope) before and after L-NAME (n=4 neurons from 4 mice, p=0.042, one-tailed paired-t test, normal distribution was assumed but not tested formally).
