Supplementary Figure 5: Formation of FTY720-P, inhibition of HDACs, and enhanced histone acetylation and gene expression in the hippocampus following oral administration of FTY720 to C57Bl/6 mice. | Nature Neuroscience

Supplementary Figure 5: Formation of FTY720-P, inhibition of HDACs, and enhanced histone acetylation and gene expression in the hippocampus following oral administration of FTY720 to C57Bl/6 mice.

From: Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory

Supplementary Figure 5

(a-e) FTY720 was administered by gavage to C57Bl/6 mice at the indicated doses. 24 h later, lymphocytes in blood were counted (a), FTY720-P levels in serum (b) and hippocampal nuclei (c) measured by LCESI-MS/MS, and hippocampus HDAC activity (d) and H3K9 acetylation (e) were determined. (f-i) QPCR analysis of Vegfd, Bdnf, and cFos expression after treatment of mice with different doses of FTY720 for 24 h (f,g) or with 0.1 mg/kg for the indicated times (h,i). *P < 0.01, compared to untreated (unpaired two-tailed t test).

Back to article page