Supplementary Figure 5: Knockdown of ALK5 by shRNA-A2 retrovirus in progenitor cells decelerates dendrite development of newborn neurons in the dentate gyrus.
From: ALK5-dependent TGF-β signaling is a major determinant of late-stage adult neurogenesis
(a,b) Brightfield immunohistochemical detection of virus-infected eGFP-immunostained cells (left) and confocal images of eGFP, DCX, and DAPI triple-labeled newborn neurons (right) in the dentate gyrus of stereotaxically injected 2-month-old C57BL/6 mice at 7 (a) and 14 dpi (b). Scale bar is 50 μm in brightfield immunohistochemical images and 20 μm in confocal images. (c) Quantification of dendritic length of virus-infected newborn neurons in the dentate gyrus at 7 dpi. (d,e) Quantification of dendritic length (d) and number (e) of virus-infected newborn neurons in the dentate gyrus at 14 dpi. n = 17, 12 neurons from two mice total for each group at 7 dpi. n = 13, 9 neurons from two mice total for each group at 14 dpi. Data are presented as mean + s.d. **P < 0.01, ***P < 0.001, Student's t-test.
